Produção Científica http://repositoriobiologico.com.br//jspui/handle/123456789/1 2026-07-07T20:00:26Z 2026-07-07T20:00:26Z Riquelme, Guilherme Mineiro, Jeferson Luiz de Carvalho Sato, Mário Eidi http://repositoriobiologico.com.br//jspui/handle/123456789/1320 2026-07-07T15:15:49Z 2025-01-01T00:00:00Z

Title: Predation and oviposition performance of Amblydromalus limonicus when fed with different life stages of Brevipalpus yothersi Authors: Riquelme, Guilherme; Mineiro, Jeferson Luiz de Carvalho; Sato, Mário Eidi Abstract: Citrus leprosis is one of the most critical diseases in citrus orchards in Brazil. The use of predatory mites from the family Phytoseiidae may represent a promising alternative for controlling Brevipalpus yothersi, the main vector of the citrus leprosis virus. The study was carried out to evaluate the potential use of Amblydromalus limonicus for the biological control of B. yothersi, verifying predation rates on different life stages (eggs, larvae, and adults) of leprosis mite and observing predator oviposition rates resulting from its feeding behaviour. Description: Citrus leprosis is one of the most critical diseases in citrus orchards in Brazil. The use of predatory mites from the family Phytoseiidae may represent a promising alternative for controlling Brevipalpus yothersi, the main vector of the citrus leprosis virus. The study was carried out to evaluate the potential use of Amblydromalus limonicus for the biological control of B. yothersi, verifying predation rates on different life stages (eggs, larvae, and adults) of leprosis mite and observing predator oviposition rates resulting from its feeding behaviour. The predator showed a high predation rate for eggs and larvae of B. yothersi, consuming in average 76.8 eggs and 69.5 larvae of the mite per predator female per day, for the highest prey density (80) per arena. This feeding resulted in oviposition rates of up to 1.75 and 2.0 eggs per female predator per day, for eggs and larvae of B. yothersi, respectively. In the case of B. yothersi adults, the predation rates were very low (≤0.25 adults per predator female per day). The results indicate that A. limonicus has potential for use as a biological control agent for the citrus leprosis mite.

2025-01-01T00:00:00Z Silva, Roberto Tadeu da http://repositoriobiologico.com.br//jspui/handle/123456789/1319 2026-07-06T13:38:34Z 2026-07-01T00:00:00Z

Title: Guia para a elaboração de dissertações e teses Authors: Silva, Roberto Tadeu da Abstract: Nesta quinta edição, o Guia para Elaboração de Dissertações e Teses foi atualizado de acordo com as diretrizes de documentação mais recentes da ABNT. Além das atualizações dos hyperlinks contidos no texto, foram incluídos esclarecimentos sobre a utilização de ferramentas de inteligência artificial e um exemplo de declaração de seu emprego. Reforçamos que as dissertações e teses podem ser redigidas no idioma inglês, promovendo a internacionalização e acessibilidade à produção científica do Instituto Biológico. Para facilitar o acesso ao conteúdo aqui apresentado, este Guia está disponível no Repositório da Produção Técnica e Científica do IB em http://repositoriobiologico.com.br/jspui/ e nos sites da biblioteca em http://www.biologico.sp.gov.br/page/produtos-e-servicos/biblioteca e da Pós-Graduação em https://biologico.agricultura.sp.gov.br/pos/#/page/dissertacao-instrucoes. Dúvidas e sugestões devem ser encaminhadas para biblioteca@biologico.sp.gov.br. Description: Nesta quinta edição, o Guia para Elaboração de Dissertações e Teses foi atualizado de acordo com as diretrizes de documentação mais recentes da ABNT. Além das atualizações dos hyperlinks contidos no texto, foram incluídos esclarecimentos sobre a utilização de ferramentas de inteligência artificial e um exemplo de declaração de seu emprego. Reforçamos que as dissertações e teses podem ser redigidas no idioma inglês, promovendo a internacionalização e acessibilidade à produção científica do Instituto Biológico. Para facilitar o acesso ao conteúdo aqui apresentado, este Guia está disponível no Repositório da Produção Técnica e Científica do IB em http://repositoriobiologico.com.br/jspui/ e nos sites da biblioteca em http://www.biologico.sp.gov.br/page/produtos-e-servicos/biblioteca e da Pós-Graduação em https://biologico.agricultura.sp.gov.br/pos/#/page/dissertacao-instrucoes. Dúvidas e sugestões devem ser encaminhadas para biblioteca@biologico.sp.gov.br.

2026-07-01T00:00:00Z Riquelme, Guilherme Martins Gomes de Castro, Tatiane Marie Carvalho Mineiro, Jeferson Luiz de Sato, Mario Eidi http://repositoriobiologico.com.br//jspui/handle/123456789/1318 2026-04-30T17:39:52Z 2025-01-01T00:00:00Z

Title: First report of the genus Parabonzia Smilley Authors: Riquelme, Guilherme; Martins Gomes de Castro, Tatiane Marie; Carvalho Mineiro, Jeferson Luiz de; Sato, Mario Eidi Abstract: We report the predatory mite Parabonzia xinningensis Chen & Jin, 2020 (Acari: Cunaxidae), collected from leaves of Citrus sinensis (L.) Osbeck (Rutaceae) in the municipality of Mogi Mirim, state of São Paulo, Brazil. This is the first record of a mite of the genus Parabonzia for the country and for South America. Description: Cunaxidae (Acari: Prostigmata) is composed exclusively of free-living predatory mites, which feed on a wide range of organisms, such as other mites, insects, nematodes and fungi (Walter and Kaplan 1991; Den Heyer 2009). Currently, the family is comprised of 463 species distributed in 32 genera worldwide (Skvarla 2025). Most of South America has not been surveyed for Cunaxidae and diversity in many areas is unknown. However, Brazil has received the most attention, with 22 of the worldwide genera recorded in the country (Wurlitzer et al. 2020; Wurlitzer et al. 2021; Rocha and Ferla 2025).

2025-01-01T00:00:00Z Ninga, Ederina Sapozhnikova, Sapozhnikova Lehotay, Steven J Lightfield, Alan R Monteiro, Sergio H http://repositoriobiologico.com.br//jspui/handle/123456789/1317 2026-02-06T17:14:16Z 2020-05-01T00:00:00Z

Title: High-Throughput Mega-Method for the Analysis of Pesticides, Veterinary Drugs, and Environmental Contaminants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Robotic Mini-Solid-Phase Extraction Cleanup + Low-Pressure Gas Chromatography-Tandem Mass Spectrometry, Part 2: Catfish Authors: Ninga, Ederina; Sapozhnikova, Sapozhnikova; Lehotay, Steven J; Lightfield, Alan R; Monteiro, Sergio H Abstract: The goal of this study was to develop and validate a new method for simultaneous determination of 106 veterinary drugs and 227 pesticides and their metabolites plus 16 polychlorinated biphenyls (PCBs) at and below their regulatory levels established for catfish muscle in the European Union and U.S.A. To do this, two different QuEChERS-based methods for veterinary drugs and pesticides and PCBs were modified and merged into a single mega-method dubbed “QuEChERSER” (more than QuEChERS), which is presented here for the first time. The mega-method was validated in catfish at four different spiking levels with 10 replicates per level. Sample extraction of 2 g test portions was made with 10 mL of 4:1 (v/v) acetonitrile/water, and then an aliquot was taken for ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) analysis of 106 veterinary drugs and 125 pesticides, including metabolites. The remaining extract after salting out was subjected to automated mini-solid-phase extraction cleanup (Instrument Top Sample Preparation) for immediate injection in low-pressure gas chromatography–tandem mass spectrometry (LPGC–MS/MS). The cleanup was conducted in parallel with the 10 min LPGC–MS/MS analysis for 167 PCBs, pesticides, and metabolites, which was conducted in parallel with the 10 min UHPLC–MS/MS analysis for 231 analytes to increase sample throughput (49 analytes were included in both techniques). In MS/MS, three ion transitions were monitored for nearly all targeted analytes to provide unambiguous identification as well as quantification. Satisfactory recoveries (70–120%) and relative standard deviations of ≤20% were achieved for 98 (92%) of the veterinary drugs and their metabolites and for 222 (91%) of pesticides, metabolites, and PCBs, demonstrating that the developed method is applicable for the analysis of these contaminants in fish as part of regulatory monitoring programs and other purposes. Description: The goal of this study was to develop and validate a new method for simultaneous determination of 106 veterinary drugs and 227 pesticides and their metabolites plus 16 polychlorinated biphenyls (PCBs) at and below their regulatory levels established for catfish muscle in the European Union and U.S.A. To do this, two different QuEChERS-based methods for veterinary drugs and pesticides and PCBs were modified and merged into a single mega-method dubbed “QuEChERSER” (more than QuEChERS), which is presented here for the first time. The mega-method was validated in catfish at four different spiking levels with 10 replicates per level. Sample extraction of 2 g test portions was made with 10 mL of 4:1 (v/v) acetonitrile/water, and then an aliquot was taken for ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) analysis of 106 veterinary drugs and 125 pesticides, including metabolites. The remaining extract after salting out was subjected to automated mini-solid-phase extraction cleanup (Instrument Top Sample Preparation) for immediate injection in low-pressure gas chromatography–tandem mass spectrometry (LPGC–MS/MS). The cleanup was conducted in parallel with the 10 min LPGC–MS/MS analysis for 167 PCBs, pesticides, and metabolites, which was conducted in parallel with the 10 min UHPLC–MS/MS analysis for 231 analytes to increase sample throughput (49 analytes were included in both techniques). In MS/MS, three ion transitions were monitored for nearly all targeted analytes to provide unambiguous identification as well as quantification. Satisfactory recoveries (70–120%) and relative standard deviations of ≤20% were achieved for 98 (92%) of the veterinary drugs and their metabolites and for 222 (91%) of pesticides, metabolites, and PCBs, demonstrating that the developed method is applicable for the analysis of these contaminants in fish as part of regulatory monitoring programs and other purposes.

2020-05-01T00:00:00Z