http://repositoriobiologico.com.br//jspui/handle/123456789/37 2026-03-11T14:13:32Z 2026-03-11T14:13:32Z BABESCO, C.E. OLIVEIRA, A.A. CAMPOS, A.E.C http://repositoriobiologico.com.br//jspui/handle/123456789/1242 2025-02-12T18:13:10Z 2025-02-01T00:00:00Z

Title: Estrutura de comunidade das formigas urbanas do bairro da Vila Mariana na cidade de São Paulo: uma análise temporal Authors: BABESCO, C.E.; OLIVEIRA, A.A.; CAMPOS, A.E.C Abstract: As formigas são insetos sociais e ocorrem, praticamente, em todos os ambientes terrestres, exceto nos polos. No Brasil, embora tendo sido catalogadas cerca de 2.000 espécies destes artrópodes, apenas entre 20 a 30 delas são consideradas pragas urbanas. Apesar de diversas espécies apresentarem uma distribuição quase mundial, ainda são escassos os estudos que aprofundam o conhecimento acerca de seus impactos como pragas na América Latina especialmente análises temporais para entender as comunidades de formigas ao longo do tempo. Description: As formigas são insetos sociais e ocorrem, praticamente, em todos os ambientes terrestres, exceto nos polos. No Brasil, embora tendo sido catalogadas cerca de 2.000 espécies destes artrópodes, apenas entre 20 a 30 delas são consideradas pragas urbanas. Apesar de diversas espécies apresentarem uma distribuição quase mundial, ainda são escassos os estudos que aprofundam o conhecimento acerca de seus impactos como pragas na América Latina especialmente análises temporais para entender as comunidades de formigas ao longo do tempo. Esse estudo teve por objetivos reavaliar a estrutura de comunidades de formigas no Bairro da Vila Mariana - São Paulo após 10 e 20 anos; verificar se existem associações entre os padrões de conservação e limpeza da residência com os graus de infestação encontrados; analisar se existem associações positivas entre as características estruturais da residência, presença de animais, de crianças e uso de inseticida e a ocorrência de formigas.

2025-02-01T00:00:00Z Monteiro, Sérgio H Barbosa, C.M Delmondes, Beatriz S.D Silva, Julio C.C Gonçalves, José A Andrade, Graziela M http://repositoriobiologico.com.br//jspui/handle/123456789/1241 2024-11-01T11:45:39Z 2024-01-01T00:00:00Z

Title: One pot extraction method for multiresidue and polar pesticides analysis: green chemistry approach cutting time and costs. Authors: Monteiro, Sérgio H; Barbosa, C.M; Delmondes, Beatriz S.D; Silva, Julio C.C; Gonçalves, José A; Andrade, Graziela M Abstract: Sample preparation methods can pose challenges depending on the physicochemical properties of the pesticides under analysis. Polar compounds, exhibiting higher polarity compared to those detected in multiresidue methods, present unique hurdles such as lower sensitivity, matrix interferents, and the requirement of chromatographic columns with diverse retention mechanisms for detection when it does not use the derivatization approach. In this study, we successfully extracted polar pesticides alongside those typically analyzed in multiresidue methods using a single extraction solvent of acetonitrile and water. For polar compounds like glyphosate, glufosinate, AMPA, fosetyl-Al, maleic hydrazine, and ethephon, a 500 μL aliquot was filtered and analyzed using an Acclaim Trinity Q1 column (2.1 x 100 mm, 3 μm). Subsequently, QuEChERS EN 15662 extraction salts were added to the same centrifuge tube for partitioning and cleaned up with PSA. This facilitated the preparation of samples for determination of other compounds by GC-MS/MS and UPLC-MS/MS. Liquid chromatography coupled with tandem mass spectrometry was employed for detecting both polar and multiresidue pesticides. Although extra caution was needed, such as periodic cleaning runs for the column. The polar method was validated with accuracy ranging between 86% and 129%, and coefficients of variation (CV%) below 19% interday at 0.05 mg/kg, and between 67% and 111% (CV% 18) interday at 0.2 mg/kg. Verification of an additional 273 pesticides yielded accuracy rates of 52% to 137% (CV% 20.3) at 0.02 mg/kg. Sharing the extraction method led to reduced preparation time, solvent consumption, and streamlined laboratory workflows. Overall, the results underscore the efficacy of this extraction method, offering a faster and more costeffective alternative for integrating polar compounds into laboratory routines. Description: Sample preparation methods can pose challenges depending on the physicochemical properties of the pesticides under analysis. Polar compounds, exhibiting higher polarity compared to those detected in multiresidue methods, present unique hurdles such as lower sensitivity, matrix interferents, and the requirement of chromatographic columns with diverse retention mechanisms for detection when it does not use the derivatization approach.

2024-01-01T00:00:00Z Andrade, Graziela M. Delmondes, Beatriz S.D. Silva, Julio C.C. Gonçalves, José A. Monteiro, Sérgio H. http://repositoriobiologico.com.br//jspui/handle/123456789/1240 2024-10-31T19:10:48Z 2024-01-01T00:00:00Z

Title: Food processing factor to glyphosate in bean after application of different processing techniques Authors: Andrade, Graziela M.; Delmondes, Beatriz S.D.; Silva, Julio C.C.; Gonçalves, José A.; Monteiro, Sérgio H. Abstract: Beans are a valuable source of vegetable protein and essential nutrients. Brazil is one of the world's largest producers of beans, with a significant portion of the national production earmarked for domestic consumption, owing to the high demand for this food in the Brazilian diet. The food processing factor is an important consideration in managing risks associated with consuming contaminated foods. It refers to the reduction or concentration of pesticide residues during food processing, such as washing, peeling, cooking, and other culinary techniques. In this study, a method was developed for the analysis of polar compounds (glyphosate, glufosinate, AMPA, fosetyl-Al, maleic hydrazine, and ethephon) in bean samples using UPLC-MS/MS and an Acclaim Trinity Q1 column (2.1 x 100 mm, 3 μm) for chromatographic separation (One Pot Extraction Method for Multiresidue and Polar Pesticides Analysis). Furthermore, an investigation was conducted to assess whether different soaking conditions (no soaking, soaking in water, and soaking in water with 1% vinegar) – Factor A, and cooking methods (conventional pot, microwave, and pressure cooker) – Factor B, affect the glyphosate concentration in naturally contaminated bean samples through a 3^2 factorial analysis. There was no difference in variance between the samples, and in the ANOVA test, Factor A (type of soak) showed no significant difference, while Factor B (type of pot) had a significant effect on glyphosate loss. The experiment involving cooking beans in a pressure cooker with a 12-hour soak resulted in a 77.1% loss of glyphosate. Conversely, beans cooked in a regular pot (90 min) with a 1% vinegar soak experienced a 52.7% glyphosate loss in sample beans. The research findings confirmed that cooking processes significantly reduced the concentration of glyphosate in beans. However, soaking them did not affect the reduction. The food processing factor ranged from 0.23 to 0.47, with the pressure cooker experiment showing the lowest values and the highest glyphosate degradation under high pressure and temperature, within the shortest cooking time (40 min) among the treatments. Description: Beans are a valuable source of vegetable protein and essential nutrients. Brazil is one of the world's largest producers of beans, with a significant portion of the national production earmarked for domestic consumption, owing to the high demand for this food in the Brazilian diet. The food processing factor is an important consideration in managing risks associated with consuming contaminated foods. It refers to the reduction or concentration of pesticide residues during food processing, such as washing, peeling, cooking, and other culinary techniques.

2024-01-01T00:00:00Z