Use este identificador para citar ou linkar para este item: http://repositoriobiologico.com.br//jspui/handle/123456789/165
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorSantander Parra, Silvana-
dc.contributor.authorNunez, Luis-
dc.contributor.authorBuim, Marcos Roberto-
dc.contributor.authorAstolfi-Ferreira, Claudete Serrano-
dc.contributor.authorPiantino Ferreira, Antônio José-
dc.date.accessioned2020-04-02T17:47:55Z-
dc.date.available2020-04-02T17:47:55Z-
dc.date.issued2018-07-23-
dc.identifier.citationSantander Parra, Silvana; Nunez, Luis; Buim, Marcos Roberto; Astolfi-Ferreira, Claudete Serrano; Piantino Ferreira, Antonio José. Development of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE gene. British Poultry Science. v. 59, n. 4, p. 402-407, 2018.pt_BR
dc.identifier.issn1466-1799pt_BR
dc.identifier.urihttp://repositoriobiologico.com.br//jspui/handle/123456789/165-
dc.description.abstractInfectious laryngotracheitis is a respiratory disease that affects the poultry industry worldwide. It is common in flocks with high-bird density, causing major economic losses. 2. In this study, a SYBR® FAST polymerase chain reaction (PCR) double-strand DNA intercalating agent assay was performed for the detection of infectious laryngotracheitis virus (ILTV) in clinical samples in comparison with a conventional nested-PCR, both based on the glycoprotein E encoding gene. This assay amplified 56 bp and was capable of detecting 19 to 1 copies of virus. 3. In total, 164 clinical samples were obtained from birds with respiratory problems from the period of 2009-2016. In the nested-PCR, there were 45.12% positive samples and 54.88% negative samples, while in the real-time PCR (qPCR), there were 81.1% positive samples and 18.9% negative samples. 4. In conclusion, qPCR from the DNA double-strand intercalating agent SYBR® GREEN FAST was useful for the diagnosis of ILTV because it detected samples that were negative in nested-PCR. This assay has advantages, such as a shortened processing-time, and no need for post-amplification processing (electrophoresis) with additional reagents, such as MgCl2 and agarose. Hence, qPCR proved to be useful, rapid and low cost for use with clinical samples.pt_BR
dc.description.sponsorshipFAPESPpt_BR
dc.language.isoen_USpt_BR
dc.subjectChickenspt_BR
dc.subjectDetectionpt_BR
dc.subjectgE genept_BR
dc.subjectInfectious laryngotracheitispt_BR
dc.subjectNested-PCRpt_BR
dc.subjectqPCRpt_BR
dc.titleDevelopment of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE genept_BR
dc.identifier.doi10.1080/00071668.2018.1479060pt_BR
dc.identifier.tipoRegulamentadopt_BR
Aparece nas coleções:Artigos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.