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Título: Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana
Título(s) alternativo(s): Atividade antimicrobiana e antibiofilme do óleo essencial de folhas secas de Eucalyptus staigeriana
Autor(es): Correa, Marcos Saldanha
Schwambach, Joseli
Mann, Michele Bertoni
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
Data do documento: 14-Jan-2019
Resumo: In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no reports of antimicrobial activity of essential oils extracted from dried leaves of Eucalyptus staigeriana. So, the objectives of this study were to determine the chemical composition of the essential oils obtained from dried leaves of E. staigeriana (EOdlES) and to evaluate in vitro antimicrobial and antibiofilm activities of EOdlES against gram-positive and gram-negative, resistance and multiresistant Enterococcus faecalis isolated from food and clinical samples. The characterization of EOdlES was performed by gas chromatography-mass spectrometry (GC/MS). For this study, 26 bacterial strains were used, which included 11 reference strains and 15 antibiotic resistant and multiresistant E. faecalis strains. Antimicrobial activities of EOdlES against gram-positive and gram-negative were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) value was evaluated by a microbroth dilution technique. The antibiofilm effects were assessed by microtiter plate method. As a result, 21 compounds were identified, being oxygenated monoterpenes (69.58%) the major chemical family. EOdlES showed only antimicrobial activity against gram-positive strains. E. faecalis resistant and multiresistant strains show the lowest MIC (3.12 to 6.25%), when compared with reference E. faecalis strain. EOdlES has the ability to inhibit the biofilm formation, but little or none ability to inhibit the preformed biofilm. This study demonstrates that EOdlES is a promising alternative to control important foodborne and clinic gram-positive resistant bacteria.
Palavras-chave: Antimicrobial activity
Antibiofilm activit
Essential oils
Dried leaves
Antibiotic-resistant strains
Eucalyptus staigeriana
Citação: Correa, Marcos Saldanha; Schwambach, Joseli; Mann, Michele Bertoni; Frazzon, Jeverson; Frazzon, Ana Paula Guedes. Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana. Arquivos do Instituto Biológico. v. 86, e0202018, 2019.
ISSN: 1808-1657
Número DOI: 10.1590/1808-1657000202018
Idioma: en_US
Editora: Instituto Biológico
Local da Publicação: São Paulo
URI: http://repositoriobiologico.com.br//jspui/handle/123456789/377
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