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Título: | Performance assessment of imported ELISA in the serodiagnosis of the enzootic bovine leukosis in herds of Pernambuco state, Brazil* |
Título(s) alternativo(s): | Avaliação do desempenho de ELISA importado no sorodiagnóstico da leucose enzoótica bovina em rebanhos do estado de Pernambuco, Brasil |
Autor(es): | Baptista Filho, Luiz Carlos Fontes Fernandes, Luiz Carlos Fontes Silva, Tamyres Izarelly Barbosa da Ramos, Taciana Rabelo Ramalho Melo, Lúcio Esmeraldo Honório de |
Data do documento: | 25-Nov-2019 |
Resumo: | Enzootic bovine leukosis (EBL) is an infectious disease of cosmopolitan distribution and chronic character caused by a virus of the Retroviridae family, bovine leukemia virus (BLV). The epidemiological situation of EBL in Brazil has motivated studies to improve its diagnosis, based on the recommended serological techniques: agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA). This study was designed to evaluate the use of imported ELISA for the detection of BLV in dairy herds raised in Pernambuco, Brazil, comparing it to AGID. Blood serum samples from 327 dairy cattle from the state of Pernambuco were tested to AGID and the imported commercial ELISA CHEKIT-Leucose-serum, produced by the IDEXX® laboratory for the diagnosis of EBL. Discarding 25 inconclusive samples from one or both tests, 302 samples were analyzed, being 24.1% positive (73/302) in the AGID and 45% (136/302) in the ELISA, which compared to the AGID, a technique considered standard, presented sensitivity of 98.6%, specificity of 72% and Kappa coefficient of 0.55. The lack of agreement in the diagnostic methods was probably due to the high sensitivity of the ELISA, which makes it possible to detect antibodies even in situations with low serum levels. Although AGID has been shown to be an efficient test so far, in more advanced stages of an EBL control and eradication program, with low prevalence rates, ELISA will present better performance, due to its higher sensitivity, avoiding the permanence of animals that spread the disease in the herds. |
Palavras-chave: | Cattle Bovine leukemia virus Bovine leukemia virus AGID Serology |
Citação: | Baptista Filho, Luiz Carlos Fontes; Fernandes, Luiz Carlos Fontes; Silva, Tamyres Izarelly Barbosa da; Ramos, Taciana Rabelo Ramalho; Melo, Lúcio Esmeraldo Honório de. Performance assessment of imported ELISA in the serodiagnosis of the enzootic bovine leukosis in herds of Pernambuco state, Braz. Arquivos do Instituto Biológico, v. 86, e0662018, 2019. |
ISSN: | 1808-1657 |
Número DOI: | 10.1590/1808-1657000662018 |
Idioma: | en_US |
Editora: | Instituto Biológico |
Local da Publicação: | São Paulo |
URI: | http://repositoriobiologico.com.br//jspui/handle/123456789/497 |
Aparece nas coleções: | Artigos |
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Arquivo | Descrição | Tamanho | Formato | |
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Performance assessment of imported ELISA.pdf | 207.04 kB | Adobe PDF | Visualizar/Abrir |
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