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Título: In vitro evaluation of aflatoxin B1 adsorption by commercial products used in animal feed
Título(s) alternativo(s): Avaliação in vitro da adsorção de aflatoxina B1 por produtos comerciais utilizados na alimentação animal
Autor(es): Pinheiro, Raizza Eveline Escórcio
Pereyra, Carina Maricel
Neves, Josyanne Araújo
Calvet, Rodrigo Maciel
Santos, Julliet Teixeira de Oliveira
Lima, Cristiane Evangelista
Alves, Verbena Carvalho
Pereira, Maria Marlucia Gomes
Muratori, Maria Christina Sanches
Data do documento: 12-Mai-2017
Resumo: The aim of this study was to evaluate the aflatoxin B1 (AFB1) adsorption capacity, in vitro, by commercial products used in animal feed. Many studies are being conducted for the decontamination of aflatoxins in feed. The commercial products destined to fish feed that are available as probiotics and are formed by strains of bacteria and yeasts used in most mycotoxins adsorption assays. Three commercial products were studied: A, consisting of Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium and Lactobacillus acidophilus; B, consisting of dry yeast of Saccharomyces cerevisiae from brewery; and C, consisting of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus. Five suspensions of the maximum dose recommended by the manufacturer of each product (0; 25; 50; 75 and 100%) were tested against AFB1 (1000 ng.mL-1) in microtubes to determine the adsorption capacity. To simulate the pH of the stomach and intestine of the Nile tilapia (Oreochromis niloticus), phosphate buffered saline solutions (PBS) at pH 1.5 and 7.5, respectively, were formulated. Microtubes were introduced into a centrifuge with mechanical agitation at 37ºC for 1 h and then centrifuged for 10 min at 14.000 rpm; the supernatants were quantified by high-performance liquid chromatography. The commercial products in the maximum concentration were capable of adsorbing AFB1 in amounts from 45.01 to 129.59; from 123.90 to 215.59; and from 209.98 to 370.73 (ng.mL-1), respectively. It was concluded that all commercial products, which are added to animal feed, adsorbed AFB1 under simulated gastrointestinal pH conditions and are potential candidates for AFB1 adsorption for future in vivo studies.
Palavras-chave: Bacillus sp.
Lactic acid bacteria
Detoxification
Saccharomyces cerevisiae
Nile tilapia
Citação: Pinheiro, Raizza Eveline Escórcio; Pereyra, Carina Maricel; Neves, JosyanneAraújo; Calvet, Rodrigo Maciel; Santos, Julliet Teixeira de Oliveira; Lima, Cristiane Evangelista;Alves, Verbena Carvalho;Pereira, Maria Marlucia Gomes;Muratori, Maria Christina Sanches. In vitro evaluation of aflatoxin B1 adsorption by commercial products used in animal feed. Arquivos do Instituto Biológico, v. 84, e0072015, 2016.
ISSN: 1808-1657
Número DOI: 10.1590/1808-1657000072015
Idioma: en_US
Editora: Instituto Biológico
Local da Publicação: São Paulo
URI: http://repositoriobiologico.com.br//jspui/handle/123456789/674
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