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dc.contributor.authorNassar, Alessandra Figueiredo de Castro-
dc.contributor.authorDaniel, Gabriela Terezinha-
dc.contributor.authorMiyashiro, Regina-
dc.contributor.authorScannapieco, Eloísa Maria-
dc.contributor.authorSouza Neto, Juraci de-
dc.contributor.authorGregory, Lilian-
dc.date.accessioned2021-03-01T01:52:46Z-
dc.date.available2021-03-01T01:52:46Z-
dc.date.issued2016-01-08-
dc.identifier.citationNassar, Alessandra Figueiredo de Castro; Daniel, Gabriela Terezinha;Miyashiro, Regina;Scannapieco, Eloísa Maria;Souza Neto, Juraci de;Gregory, Lilian. Diagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samples. Arquivos do Instituto Biológico, v. 82, 00215, 2015.pt_BR
dc.identifier.issn1808-1657pt_BR
dc.identifier.urihttp://repositoriobiologico.com.br//jspui/handle/123456789/691-
dc.description.abstractCaseous lymphadenitis is a chronic infectious disease caused by Corynebacterium pseudotuberculosis , which is a bacterium responsible for a great number of economic losses on goat and sheep production. It is characterized by the formation of abscesses in superficial lymph nodes and in internal organs and lymph nodes. This study aimed at determining the agreement between microbiological culture and PCR in the identification of C. pseudotuberculosis , in samples collected from animals in slaughterhouses and in animals that presented lymph node enlargement in field conditions. From the 202 samples analyzed through microbiological culture, 113 (56%) were positive for Corynebacterium sp.; from these positive samples, 38 (34%) were identified as C. pseudotuberculosis by microbiological culture. From the amount of samples, 110 (54%) were positive and 92 (46%) were negative in the PCR. Kappa index (0.193) presented a weak agreement between PCR and microbiological culture. We concluded that molecular diagnosis (PCR) in clinical samples proved to be more efficient, reproducible, and faster than the microbiological culture, both on clinical samples analyses and in the confirmation of C. pseudotuberculosis in colonies that were classified by Corynebacterium genus. Thus, the present study demonstrated the importance of PCR to confirm C. pseudotuberculosis diagnosis, and the best contribution for the epidemiological surveillance of the disease in sheep.pt_BR
dc.language.isoen_USpt_BR
dc.subjectC. pseudotuberculosispt_BR
dc.subjectSheeppt_BR
dc.subjectMicrobiological culturept_BR
dc.subjectPCRpt_BR
dc.titleDiagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samplespt_BR
dc.title.alternativeComparação diagnóstica de Corynebacterium pseudotuberculosis pelo cultivo microbiológico e PCR em amostras ovinaspt_BR
dc.identifier.doi10.1590/1808-1657000692013pt_BR
dc.description.editoraInstituto Biológicopt_BR
dc.description.localdapublicacaoSão Paulopt_BR
dc.identifier.tipoAbertopt_BR
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