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dc.contributor.authorElizei, Virgínia Guerra-
dc.contributor.authorChalfou, , Sara Maria-
dc.contributor.authorSantos, Botelho, Deila Magna dos-
dc.contributor.authorRebelles, Pedro Paulo Reis-
dc.date.accessioned2021-04-27T22:00:04Z-
dc.date.available2021-04-27T22:00:04Z-
dc.date.issued2014-06-
dc.identifier.citationElizei, Virgínia Guerra; Chalfou,, Sara Maria; Santos, Botelho, Deila Magna dos;Rebelles, Pedro Paulo Reis. Immobilization of filamentous fungi with potential for agribusiness. Arquivos do Instituto Biológico, v. 81(2), 165-172, 2014.pt_BR
dc.identifier.issn1808-1657pt_BR
dc.identifier.urihttp://repositoriobiologico.com.br//jspui/handle/123456789/762-
dc.description.abstractCellular immobilization represents an alternative for the bioprocess conduction, in which the cells are kept in a matrix and can be used over long periods. The objective of this work was to test a new fungi immobilization methodology with reduced cost to evaluate the viability of these fungi when submitted to the new encapsulation method, and to determine the ideal temperature to store the immobilized fungi. The mycelium of the fungi Aspergillus niger, Cladosporium cladosporioides and Penicillium solitum were mixed with 15 g of titrated rice and 3 g of sodium alginate, which was dripped in a 0.25 M calcium chloride solution for the formation of pellets. After drying in an oven at 26ºC, the granules were stored at three temperatures: room, refrigerator and freezer. The platings were carried out every 15 days in culture medium. The evaluations of the colony size and sporulation were carried out 7, 14 and 12 days after plating, for 195 days for A. niger, 225 days for C. cladosporioides, and 210 days for P. solitum. Storage temperature did not affect the mycelial development of A. niger and P. solitum. However, sporulation was reduced for the granules stored in the freezer. The mycelial development of C. cladosporioides was influenced by temperature. The granules conserved at room temperature had lower viability than those stored in the refrigerator and freezer. In the Scanning Electronic Microscopy analysis, it was observed that the immobilization is a safe method in which the fungus mycelium remains inside the granule, facilitating transport, storage and application of micro-organisms.pt_BR
dc.language.isoen_USpt_BR
dc.subjectCellular immobilizationpt_BR
dc.subjectEncapsulation of fungipt_BR
dc.subjectSodium alginatept_BR
dc.subjectGranulated formulationpt_BR
dc.titleImmobilization of filamentous fungi with potential for agribusinesspt_BR
dc.title.alternativeImobilização de fungos filamentosos com potencial para uso agroindustrialpt_BR
dc.identifier.doi10.1590/1808-1657001032012pt_BR
dc.description.editoraInstituto Biológicopt_BR
dc.description.localdapublicacaoSão Paulopt_BR
dc.identifier.tipoAbertopt_BR
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